Db Lab Report - 847 Words

The repair of DSBs NHEJ initiates when the DSBs is first recognized by the Ku heterodimer, composed of the Ku70 and Ku80 proteins and binds to the DSB in a sequence-independent manner and in doing so and protects them from nucleolytic cleavage [33, 34]. The Ku heterodimer then acts as a scaffold to recruits number NHEJ proteins including DNA-dependent protein kinase (DNA-PKcs) to the DNA ends. DNA-PKcs is a nuclear protein kinase that phosphorylates a number of protein targets, including Artemis. Once phosphorylated, Artemis forms an active endonucleolytic complex with DNA-PKcs that processes the DSB ends to make them compatible for ligation [33, 34]. Pol  µ and Pol ÃŽ » fill in the DNA gaps and lastly the XRCC4/Ligase IV complex is recruited†¦show more content†¦In the synapsis step, the Rad51-ssDNA filament (presynaptic filament) performs homology search and DNA strand invasion on a homologous region of another duplex, which results in the formation of a displacement loop (D-loop) [40]. In the post-synapsis stage of HR the invading 3†² end of the D-loop primes DNA synthesis by a DNA polymerase, which extends the D-loop enabling the second 3’ single strand terminal end of the DSB to base-pair with it - a process that is called second end capture. Once captured the second end can itself prime DNA synthesis, and the result of these â€Å"DNA transactions† is the covalent linkage of the recombining DNA molecules via two HJs. Depending on how the HJs are processed to form mature recombinant products three sub-pathways of HR are distinguished. In the canonical DSBR model the two HJs (doubleHJ/dHJ) can move along the DNA by a process called branch migration, which extends or limits the region of DNA heteroduplex formed by strand exchange catalyzed in the second step. Subsequently, dHJ intermediate could be resolved by endonucleases such as the Mus81-Eme1/Mms4 complex, Slx1-Slx4 and the GEN1/Yen1, resulting in the formation of either crossovers (CO) or non -crossover (NCO) recombinants depending on the orientation in which each HJ is cleavedShow MoreRelatedEssay on Method of Limits907 Words   |  4 PagesLab 4: Methods of limits Elizabeth Bryan Florida State University Lab 4: Method of limits. Sound waves are created when an object vibrates. They have two main components: frequency (pitch; measured in Hertz (Hz)) and amplitude (loudness, intensity; measured in decibels (dB)). 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EHRs are much more improved and structured than paper charts, allowing for a much quicker retrieval of lab or imaging results. It is also to be expected that an EHR will have an electronic troubleshootingRead MoreUses And Functions Of Oscilloscopes And The Methods Used For Measure A System s Time Constant2314 Words   |  10 PagesStatement of Objectives The objective of this lab was to gain understanding of the use and functions of oscilloscopes and the methods used to measure a system’s time constant. Several methods were used throughout the laboratory in order to gain familiarity with the equipment as well as investigate characteristics of an RC high pass filter. The ability to problem solve and critically think was necessary for completion of this laboratory and therefore an objective. Apparatus Table 1. 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Adolescence Infancy And Childhood Development - 890 Words

Infancy and Childhood Development When babies are born, their brains are ready to learn. As they learn, their brains grow. This helps them learn about the world around them. This also gives them a chance to explore the world around them in new ways. Exploring and trying things out is how babies learn. Although brain development is natural, there is a lot more to it than developing on its own. Brain development comes from moms, dads, siblings, aunts, uncles, caregivers and just the people around the babies. We help babies learn about the world around them. We give babies chances to touch, taste, see, hear, and smell as they can. We give them chances to do and learn many of things. We just have to do the right things that support brain development, such as, being caring and supportive, paying attention to the babies, giving them what they need, and providing a rich learning environment. In this essay I will focus on what babies and toddlers need from caregivers for normal brain development. Communication is key for babies and brain development. Communication sets a positive mood for babies, gives them self-confidence, independence in new task, cooperativeness and prevents them from engaging in bad behavior. Communication is important because it reflects on everyday events. This helps babies and toddlers build an internal mental life with a cultural worldview. This also prepares them for literacy. The more you speak to your children, the better vocabulary they will have. InShow MoreRelatedParenting Style Affects Our Lifelong Brain Development Essay1174 Words   |  5 Pages(1983), four parenting styles are established: authoritative, neglectful, permissive, and authoritarian. Most of us spend at least 18 years or longer with our parent(s) so it leads to a question that if parenting style affects our lifelong brain development. 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War Poetry Wilfred Owen Free Essays

War Poetry I have studied two poems, ‘Dulce et decorum est’ and ‘The Sentry’ both by the poet Wilfred Owen. The first one I will study is ‘Dulce et Decorum est’. The first thing Owen does is to give us a vivid description of what is happening, he tells us that he and his men are marching away from the trenches, and the way Owen describes his men gives us a clear picture of what they have been through. We will write a custom essay sample on War Poetry Wilfred Owen or any similar topic only for you Order Now â€Å"Bent double, like old beggars under sacks, knock-kneed, coughing like hags† They had been in the trenches, terrible places, with bullets and shells flying constantly overhead, explosions all around, the constant fear of death. These men were leaving the hell of the front, they were going to rest. But they still have a long way to go before they are safe, they are still within the range of artillery. Despite of this, they march on â€Å"towards their distant rest†, they are walking in deep mud, which covered most of the battlefield, and for this reason, there are some of the men have no boots on, but still they â€Å"limp on, blood shod†. They are described as being â€Å"drunk with fatigue†, they are exhausted, but still, they march on. Then suddenly, the call â€Å"Gas! Gas! Quick boys! â€Å", a gas shell had dropped nearby. Gas was the most brutal of weapons used in the war, it burned the skin, the eyes, and when breathed in, it burnt the lungs, which the body then filled with water, bringing on a slow agonising death for the unfortunate victim. The soldiers’ reaction to this attack is described as â€Å"an ecstasy of fumbling†, which is a great way of describing what is happening, the men are trying desperately to get out their gas masks before they are engulfed by the cloud. How to cite War Poetry Wilfred Owen, Essays

Critical Article on MacBeth Essay Example For Students

Critical Article on MacBeth Essay William Empson disagreed with many of J. M. Robertson, LiteraryDetection (1931), about certain points surrounding MacBeth. The eye winkat the hand, yet let that be which the eye fear, when tis done, to see. isa line from MacBeth which to Robertson appeared particularly vulgar. William Empson discusses this point and says that he throws out a numberof them which seem to me to sum up the thought of the play.. I agree withWilliams on this point because what Robertson passed off for being vulgar,I believe helped to sum up certain points which a scene is trying to make. For example, Robertson calls this line of MacBeth: Hover through the fogand filthy air. He even goes so far to call that a vacuous tag-line. This is a example of a line which sums up a certain point that Robertsonhas passed off as horrid. Empson points out that it establishes from thestart the theme of fog and I am within full agreement with Empson when heremarks that comment of the line. Certain lines to MacBeth, which Empson described as essential, weredisregarded by Robertson as having no sense. This paragraph shows anexample of what Robertson disregarded:But cruel are the times, when we are traitors And do not knowourselves, when we hold rumour From what we fear, yet know not what wefear, Each way and move.Robertson, after contemplating this passage, remarked that this iscertainly not Shakespeares because of the earlier point based above. Empson believes that Robertsons flaw comes within his translation of thelines, hold rumour could be like hold parley with' and goes through aretranslation of this short passage. No one who had experienced civil warcould say it had no sense. is a line which briefly sums up Robertsonsreasons for his earlier claim on this passage, his lack of experiencing acivil war. Empson does a wonderful job placing himself as the firstaudience of Shakespeare and reliving these events to their raw meaning. Ibelieve that once youve lived through a civil war with its traitors andviolent times, this passage comes through more clearly and can be seeneasier. A third point which Empson rebukes, Before my body, I throw mywarlike shield is an example of a line which Robertson remarked asadmittedly intolerable, known even by its defenders to be very bad. Robertson even goes so far as to say that (E.K.) Chambers does notdistinguish between the sense of style and the sense of sense implyingthat Chambers is not capable of examining this line fully. I believe thisline to be a powerful line showing that MacBeth is trying to protecthimself with the last of his bodily protection that he possesses. Isuspect the trouble is merely that the critics dont see the point. is aline which states clearly the problems of the previous 2 critics misleadinterpretations. William Empson has led several strong arguments againstRobertsons translation of the story MacBeth. I agree with the pointsbrought up by William Empson and believe that Robertson misinterpreted keyevents in the play of MacBeth.